Recombinant:Article Title: Allosterically inhibited PFKL via prostaglandin E2 withholds glucose metabolism and ovarian cancer invasiveness.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLAG GNI Cat#GNI4110-FG; RRID: AB_3067995 HA Cell Signaling Cat# 3724S; RRID: AB_1549585 Actin Cell Signaling Cat#3700; RRID:AB_2242334 PFKL MyBioSource Cat#MBS7048867; RRID: AB_3067998 PTGES3 R&D Cat#MAB100391; RRID: AB_3067999 5hmC Active Motif Cat#39069; RRID: AB_10013602 Chemicals, peptides, and recombinant proteins PGE2 TargetMol CatT5014 PGD2 GLPBIO Cat#GC18232 2-DG Selleck Cat#S4701 Citric acid Solarbio Cat#C8610 MMF J&K Cat#626768 MMS TCI Cat#M3262 DM-aKG Macklin Cat#D858537 DE-oxalacetate J&K Cat#587907 2-NBDG APExBIO Cat#B6035 FLAG peptide TargetMol Cat#TP1273 Fumarate hydratase-IN-1 MCE Cat#HY-100004 Critical commercial assays Glycolysis Stress Test Kit Agilent Cat#103020-100 Seahorse XFe96 FluxPaks Agilent Cat#102601–100 Fumarate Assay Kit Sigma-Aldrich Cat#MAK060-1KT Phosphofructokinase (PFK) Activity Assay Kit Solarbio Cat#BC0535 MinElute PCR Purification Kit Qiagen Cat#28004 BCA kit Beyotime Cat#P0010 rProtein A Sepharose Fast Flow GE Healthcare Cat#17-1279-01 Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17-0618-01 High-Affinity Ni-Charged Resin FF GenScript Cat#L00666-100 Deposited data Unprocessed data This study Mendeley Data: https://doi.org/10.17632/rrrj2m5zjv.3 Mass Spectrometry Proteomics This study PRIDE: PXD036495 Experimental models: Cell lines 293FT Invitrogen Cat#R70007 .. OVCAR5 Sigma-Aldrich Cat#SCC259 OVK18 Cellosaurus RRID: CVCL_3770 Oligonucleotides See Table S1 Recombinant DNA pCDH-PFKL-WT This paper N/A pCDH-PFKL-K61A This paper N/A pCDH-PFKL-K97A This paper N/A (Continued on next page) Cell Reports 42, 113246, October 31, 2023 17
Activity Assay:Article Title: Allosterically inhibited PFKL via prostaglandin E2 withholds glucose metabolism and ovarian cancer invasiveness.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLAG GNI Cat#GNI4110-FG; RRID: AB_3067995 HA Cell Signaling Cat# 3724S; RRID: AB_1549585 Actin Cell Signaling Cat#3700; RRID:AB_2242334 PFKL MyBioSource Cat#MBS7048867; RRID: AB_3067998 PTGES3 R&D Cat#MAB100391; RRID: AB_3067999 5hmC Active Motif Cat#39069; RRID: AB_10013602 Chemicals, peptides, and recombinant proteins PGE2 TargetMol CatT5014 PGD2 GLPBIO Cat#GC18232 2-DG Selleck Cat#S4701 Citric acid Solarbio Cat#C8610 MMF J&K Cat#626768 MMS TCI Cat#M3262 DM-aKG Macklin Cat#D858537 DE-oxalacetate J&K Cat#587907 2-NBDG APExBIO Cat#B6035 FLAG peptide TargetMol Cat#TP1273 Fumarate hydratase-IN-1 MCE Cat#HY-100004 Critical commercial assays Glycolysis Stress Test Kit Agilent Cat#103020-100 Seahorse XFe96 FluxPaks Agilent Cat#102601–100 Fumarate Assay Kit Sigma-Aldrich Cat#MAK060-1KT Phosphofructokinase (PFK) Activity Assay Kit Solarbio Cat#BC0535 MinElute PCR Purification Kit Qiagen Cat#28004 BCA kit Beyotime Cat#P0010 rProtein A Sepharose Fast Flow GE Healthcare Cat#17-1279-01 Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17-0618-01 High-Affinity Ni-Charged Resin FF GenScript Cat#L00666-100 Deposited data Unprocessed data This study Mendeley Data: https://doi.org/10.17632/rrrj2m5zjv.3 Mass Spectrometry Proteomics This study PRIDE: PXD036495 Experimental models: Cell lines 293FT Invitrogen Cat#R70007 .. OVCAR5 Sigma-Aldrich Cat#SCC259 OVK18 Cellosaurus RRID: CVCL_3770 Oligonucleotides See Table S1 Recombinant DNA pCDH-PFKL-WT This paper N/A pCDH-PFKL-K61A This paper N/A pCDH-PFKL-K97A This paper N/A (Continued on next page) Cell Reports 42, 113246, October 31, 2023 17
Polymerase Chain Reaction:Article Title: Allosterically inhibited PFKL via prostaglandin E2 withholds glucose metabolism and ovarian cancer invasiveness.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLAG GNI Cat#GNI4110-FG; RRID: AB_3067995 HA Cell Signaling Cat# 3724S; RRID: AB_1549585 Actin Cell Signaling Cat#3700; RRID:AB_2242334 PFKL MyBioSource Cat#MBS7048867; RRID: AB_3067998 PTGES3 R&D Cat#MAB100391; RRID: AB_3067999 5hmC Active Motif Cat#39069; RRID: AB_10013602 Chemicals, peptides, and recombinant proteins PGE2 TargetMol CatT5014 PGD2 GLPBIO Cat#GC18232 2-DG Selleck Cat#S4701 Citric acid Solarbio Cat#C8610 MMF J&K Cat#626768 MMS TCI Cat#M3262 DM-aKG Macklin Cat#D858537 DE-oxalacetate J&K Cat#587907 2-NBDG APExBIO Cat#B6035 FLAG peptide TargetMol Cat#TP1273 Fumarate hydratase-IN-1 MCE Cat#HY-100004 Critical commercial assays Glycolysis Stress Test Kit Agilent Cat#103020-100 Seahorse XFe96 FluxPaks Agilent Cat#102601–100 Fumarate Assay Kit Sigma-Aldrich Cat#MAK060-1KT Phosphofructokinase (PFK) Activity Assay Kit Solarbio Cat#BC0535 MinElute PCR Purification Kit Qiagen Cat#28004 BCA kit Beyotime Cat#P0010 rProtein A Sepharose Fast Flow GE Healthcare Cat#17-1279-01 Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17-0618-01 High-Affinity Ni-Charged Resin FF GenScript Cat#L00666-100 Deposited data Unprocessed data This study Mendeley Data: https://doi.org/10.17632/rrrj2m5zjv.3 Mass Spectrometry Proteomics This study PRIDE: PXD036495 Experimental models: Cell lines 293FT Invitrogen Cat#R70007 .. OVCAR5 Sigma-Aldrich Cat#SCC259 OVK18 Cellosaurus RRID: CVCL_3770 Oligonucleotides See Table S1 Recombinant DNA pCDH-PFKL-WT This paper N/A pCDH-PFKL-K61A This paper N/A pCDH-PFKL-K97A This paper N/A (Continued on next page) Cell Reports 42, 113246, October 31, 2023 17
Purification:Article Title: Allosterically inhibited PFKL via prostaglandin E2 withholds glucose metabolism and ovarian cancer invasiveness.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLAG GNI Cat#GNI4110-FG; RRID: AB_3067995 HA Cell Signaling Cat# 3724S; RRID: AB_1549585 Actin Cell Signaling Cat#3700; RRID:AB_2242334 PFKL MyBioSource Cat#MBS7048867; RRID: AB_3067998 PTGES3 R&D Cat#MAB100391; RRID: AB_3067999 5hmC Active Motif Cat#39069; RRID: AB_10013602 Chemicals, peptides, and recombinant proteins PGE2 TargetMol CatT5014 PGD2 GLPBIO Cat#GC18232 2-DG Selleck Cat#S4701 Citric acid Solarbio Cat#C8610 MMF J&K Cat#626768 MMS TCI Cat#M3262 DM-aKG Macklin Cat#D858537 DE-oxalacetate J&K Cat#587907 2-NBDG APExBIO Cat#B6035 FLAG peptide TargetMol Cat#TP1273 Fumarate hydratase-IN-1 MCE Cat#HY-100004 Critical commercial assays Glycolysis Stress Test Kit Agilent Cat#103020-100 Seahorse XFe96 FluxPaks Agilent Cat#102601–100 Fumarate Assay Kit Sigma-Aldrich Cat#MAK060-1KT Phosphofructokinase (PFK) Activity Assay Kit Solarbio Cat#BC0535 MinElute PCR Purification Kit Qiagen Cat#28004 BCA kit Beyotime Cat#P0010 rProtein A Sepharose Fast Flow GE Healthcare Cat#17-1279-01 Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17-0618-01 High-Affinity Ni-Charged Resin FF GenScript Cat#L00666-100 Deposited data Unprocessed data This study Mendeley Data: https://doi.org/10.17632/rrrj2m5zjv.3 Mass Spectrometry Proteomics This study PRIDE: PXD036495 Experimental models: Cell lines 293FT Invitrogen Cat#R70007 .. OVCAR5 Sigma-Aldrich Cat#SCC259 OVK18 Cellosaurus RRID: CVCL_3770 Oligonucleotides See Table S1 Recombinant DNA pCDH-PFKL-WT This paper N/A pCDH-PFKL-K61A This paper N/A pCDH-PFKL-K97A This paper N/A (Continued on next page) Cell Reports 42, 113246, October 31, 2023 17
Mass Spectrometry:Article Title: Allosterically inhibited PFKL via prostaglandin E2 withholds glucose metabolism and ovarian cancer invasiveness.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLAG GNI Cat#GNI4110-FG; RRID: AB_3067995 HA Cell Signaling Cat# 3724S; RRID: AB_1549585 Actin Cell Signaling Cat#3700; RRID:AB_2242334 PFKL MyBioSource Cat#MBS7048867; RRID: AB_3067998 PTGES3 R&D Cat#MAB100391; RRID: AB_3067999 5hmC Active Motif Cat#39069; RRID: AB_10013602 Chemicals, peptides, and recombinant proteins PGE2 TargetMol CatT5014 PGD2 GLPBIO Cat#GC18232 2-DG Selleck Cat#S4701 Citric acid Solarbio Cat#C8610 MMF J&K Cat#626768 MMS TCI Cat#M3262 DM-aKG Macklin Cat#D858537 DE-oxalacetate J&K Cat#587907 2-NBDG APExBIO Cat#B6035 FLAG peptide TargetMol Cat#TP1273 Fumarate hydratase-IN-1 MCE Cat#HY-100004 Critical commercial assays Glycolysis Stress Test Kit Agilent Cat#103020-100 Seahorse XFe96 FluxPaks Agilent Cat#102601–100 Fumarate Assay Kit Sigma-Aldrich Cat#MAK060-1KT Phosphofructokinase (PFK) Activity Assay Kit Solarbio Cat#BC0535 MinElute PCR Purification Kit Qiagen Cat#28004 BCA kit Beyotime Cat#P0010 rProtein A Sepharose Fast Flow GE Healthcare Cat#17-1279-01 Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17-0618-01 High-Affinity Ni-Charged Resin FF GenScript Cat#L00666-100 Deposited data Unprocessed data This study Mendeley Data: https://doi.org/10.17632/rrrj2m5zjv.3 Mass Spectrometry Proteomics This study PRIDE: PXD036495 Experimental models: Cell lines 293FT Invitrogen Cat#R70007 .. OVCAR5 Sigma-Aldrich Cat#SCC259 OVK18 Cellosaurus RRID: CVCL_3770 Oligonucleotides See Table S1 Recombinant DNA pCDH-PFKL-WT This paper N/A pCDH-PFKL-K61A This paper N/A pCDH-PFKL-K97A This paper N/A (Continued on next page) Cell Reports 42, 113246, October 31, 2023 17
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